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| Blay, Carole; Planes, Serge; Ky, Chin-long. |
| Nucleated pearls are produced by molluscs of the Pinctada genus through the biomineralisation activity of the pearl sac tissue within the recipient oyster. The pearl sac originates from graft tissue taken from the donor oyster mantle and its functioning is crucial in determining key factors that impact pearl quality surface characteristics. The specific role of related gene regulation during gem biogenesis was unknown, so we analysed the expression profiles of eight genes encoding nacreous (PIF, MSI60, PERL1) or prismatic (SHEM5, PRISM, ASP, SHEM9) shell matrix proteins or both (CALC1) in the pearl sac (N = 211) of Pinctada margaritifera during pearl biogenesis. The pearls and pearl sacs analysed were from a uniform experimental graft with sequential... |
| Tipo: Text |
Palavras-chave: Biomineralisation; Relative gene expression; Pearl sac; Biomarkers; Pearl quality; Pinctada margaritifera. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00435/54622/56022.pdf |
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| Ky, Chin-long; Demmer, Jonathan; Sham-koua, Manaarii; Cabral, Philippe. |
| Production of larger, rounder, high-quality cultured pearls with fewer circles is one of the main challenges of Pinctada margaritifera aquaculture faced by every pearl farm in French Polynesia. Although bigger pearl sizes can be achieved through surgreffe operations (implantation of a second nucleus following pearl harvest), control of the development of pearl circles and shapes still remains unclear, as illustrated by grafter’s empirical rules, where often the surgreffe process is only performed after production of uncircled and round pearl shapes. The present study was designed with a real pearl by pearl traceability to reveal for the first time the development of circles and shapes from graft and surgreffe, in relation to the size of the pearl sac. This... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Surgreffe; Circled pearls; Pearl shapes; Pearl sac. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38690/37187.pdf |
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| Arnaud, Sophie; Goyard, Emmanuel; Vonau, Vincent; Herbaut, C; Prou, Jean; Saulnier, Denis. |
| Most bivalves species of the genus Pinctada are well known throughout the world for production of white or black pearls of high commercial value. For cultured pearl production, a mantle allograft from a donor is implanted into the gonad of a recipient oyster, together with a small inorganic bead. Because of the dedifferentiation of cells during the first steps of the host oyster's immunological reaction, so far the fate of the graft and its exact role in the process of pearl formation could not be determined via classical histological methods. Here we report the first molecular evidence of the resilience of the graft in the recipient organism by showing that cells containing genome from the donor are still present at the end of pearl formation. These... |
| Tipo: Text |
Palavras-chave: Genotype; Pearl; Pearl sac; Grafting; Biomineralization; Pearl oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2456.pdf |
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| Le Luyer, Jeremy; Auffret, Pauline; Quillien, Virgile; Leclerc, Nicolas; Reisser, Celine; Vidal-dupiol, Jeremie; Ky, Chin-long. |
| Background Cultured pearls are unique gems produced by living organisms, mainly molluscs of the Pinctada genus, through the biomineralization properties of pearl sac tissue. Improvement of P. margaritifera pearl quality is one of the biggest challenges that Polynesian research has faced to date. To achieve this goal, a better understanding of the complex mechanisms related to nacre and pearl formation is essential and can now be approached through the use of massive parallel sequencing technologies. The aim of this study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that had producing pearls with high and low quality. For this purpose, a comprehensive reference transcriptome of P. margaritifera was built based on... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Biomineralization; Pearl quality; Pearl sac; RNA-seq. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00475/58695/61207.pdf |
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